Romanov V L, Grechkina G M, Adanin V M, Starovoĭtov I I
Mikrobiologiia. 1993 Sep-Oct;62(5):887-96.
The strain Pseudomonas aeruginosa 142 isolated from the utilising PSBs bacterial association was capable of growth on 2-chloro- and 2,4-dichlorobenzoates as sole carbon sources, but it did not utilize 3-Cl, 4-Cl, 3,5-diCl- and 2,6-dichlorobenzoates. P. aeruginosa 142 dehalogenated 2-Cl-, 2,4-diCl- and 2,5-dichlorobenzoates in aerobic conditions. The release of chloride was not observed in microaerophilic and anaerobic conditions. The activities of catechol-1,2-dioxygenase and 4-chlorocatechol-1,2-dioxygenase were found in cell extracts after growth of this strain on 2,4-dichlorobenzoate. The presented results suggested that oxidative release of chloride in ortho-position is the first step of metabolism of 2-Cl-, 2,4-diCl- and 2,5-dichlorobenzoates. The further splitting of corresponding catechols is carried out by ortho-pathway.
从利用光合细菌的细菌联合体中分离出的铜绿假单胞菌142菌株，能够以2-氯苯甲酸酯和2,4-二氯苯甲酸酯作为唯一碳源生长，但它不能利用3-氯、4-氯、3,5-二氯和2,6-二氯苯甲酸酯。铜绿假单胞菌142在有氧条件下使2-氯苯甲酸酯、2,4-二氯苯甲酸酯和2,5-二氯苯甲酸酯脱卤。在微需氧和厌氧条件下未观察到氯的释放。该菌株在2,4-二氯苯甲酸酯上生长后，在细胞提取物中发现了儿茶酚-1,2-双加氧酶和4-氯儿茶酚-1,2-双加氧酶的活性。所呈现的结果表明，邻位氯的氧化释放是2-氯苯甲酸酯、2,4-二氯苯甲酸酯和2,5-二氯苯甲酸酯代谢的第一步。相应儿茶酚的进一步裂解是通过邻位途径进行的。
