Maślanka K, Brojer E, Gromadzka G, Zupańska B
Zakład Serologii, Instytut Hematologii i Transfuzjologii, Warszawa.
Acta Haematol Pol. 1993;24(4):373-9.
The enzyme test with GPIIb/IIIa has been used to detect antibodies in 500 sera from patients with thrombocytopenia and mothers of infants with thrombocytopenia. The results were compared with antibody detection in the platelet suspension immunofluorescence test (PIFT) and in the monoclonal antibody immobilization of platelet antigens (MAIPA). Platelet antibodies were detected in 125 sera (25%). In 46.2% the results were positive in all three tests. However, in 38.4% the ELISA was negative while the PIFT and MAIPA were positive. On the other hand, in 14.4% the ELISA was positive while the PIFT and/or the MAIPA were negative. The ELISA appears to be useful in the detection of anti-HPA-1a antibodies, which are the main cause of AIMN and PTP. This test, however, seems to be less useful in the detection of autoantibodies. The significance of the ELISA in the detection of platelet alloantibodies responsible for refractoriness to platelet transfusions is not clear yet and requires further investigations.
采用GPIIb/IIIa酶试验检测了500例血小板减少症患者及血小板减少症婴儿母亲血清中的抗体。将结果与血小板悬液免疫荧光试验（PIFT）及血小板抗原单克隆抗体固定试验（MAIPA）中的抗体检测结果进行了比较。在125份血清（25%）中检测到了血小板抗体。46.2%的结果在所有三项试验中均为阳性。然而，38.4%的酶联免疫吸附测定（ELISA）结果为阴性，而PIFT和MAIPA结果为阳性。另一方面，14.4%的ELISA结果为阳性，而PIFT和/或MAIPA结果为阴性。ELISA似乎在检测抗HPA-1a抗体方面有用，抗HPA-1a抗体是同种免疫性新生儿血小板减少症（AIMN）和血小板输注无效（PTP）的主要原因。然而，该试验在检测自身抗体方面似乎用处较小。ELISA在检测导致血小板输注无效的血小板同种抗体中的意义尚不清楚，需要进一步研究。
