纸色谱法作为1,25 - 二羟基维生素D测定中制备步骤的优势。

Advantages of paper chromatography as a preparative step in the assay of 1,25-dihydroxyvitamin D.

作者信息

van Hoof H J, Swinkels L M, van Stevenhagen J J, van den Berg H, Ross H A, Benraad T J

机构信息

Department of Experimental and Chemical Endocrinology, University Hospital Nijmegen, Netherlands.

出版信息

J Chromatogr. 1993 Nov 17;621(1):33-9. doi: 10.1016/0378-4347(93)80073-d.

DOI:10.1016/0378-4347(93)80073-d

PMID:8308085

Abstract

Ether extraction and paper chromatography were used to separate the main metabolites of vitamin D in plasma [25-(OH), 24,25-(OH)2 and 1,25-(OH)2 vitamin D]prior to radio receptor-assay. The overall procedural loss of the 1,25-(OH)2 vitamin D was 58 +/- 5% (n = 40), corrected for by tracer addition. The sensitivity of the assay was 0.5 fmol/tube, corresponding to 4 pmol/l, and the intra- and inter-assay coefficients of variation were 10.5% and 11.5%, respectively. The range of values measured in healthy controls was 80-200 pmol/l (n = 60), which is in agreement with findings reported in the literature. A comparison of the results of the present procedure with those obtained with a procedure employing C18 purification, disclosed a correlation coefficient of 0.92 (p < or = 0.0001), a slope of 0.89 (p < or = 0.0001) and a small non-significant intercept of 5.0 pmol/l (n = 53).

摘要

在进行放射受体测定之前，采用乙醚萃取和纸色谱法分离血浆中维生素D的主要代谢产物[25 -（OH）、24,25 -（OH）₂和1,25 -（OH）₂维生素D]。通过添加示踪剂校正后，1,25 -（OH）₂维生素D的总体程序损失为58±5%（n = 40）。该测定方法的灵敏度为0.5 fmol/管，相当于4 pmol/L，批内和批间变异系数分别为10.5%和11.5%。健康对照者测得的值范围为80 - 200 pmol/L（n = 60），这与文献报道的结果一致。将本方法的结果与采用C18纯化方法获得的结果进行比较，发现相关系数为0.92（p≤0.0001），斜率为0.89（p≤0.0001），截距为5.0 pmol/L，无显著性差异（n = 53）。