存档人体组织中白细胞介素-4 mRNA的非同位素原位检测

Non-isotopic in situ detection of mRNA for interleukin-4 in archival human tissue.

作者信息

Bromley L, McCarthy S P, Stickland J E, Lewis C E, McGee J O

机构信息

Nuffield Department of Pathology and Bacteriology, University of Oxford, John Radcliffe Hospital, Headington, UK.

出版信息

J Immunol Methods. 1994 Jan 3;167(1-2):47-54. doi: 10.1016/0022-1759(94)90073-6.

DOI:10.1016/0022-1759(94)90073-6

PMID:8308286

Abstract

Non-isotopic in situ hybridisation (NISH) is a rapid and sensitive method currently used in molecular pathology to identify DNA in fresh and archival human biopsies. In this study we have extended NISH technology for mRNA detection to include the use of digoxigenin-labelled riboprobes to localise low abundance mRNA for interleukin-4 in paraffin embedded material (from active inflammatory bowel disease). As this methodological approach makes the retrospective study of cytokine gene expression in archival material possible for the first time, it could act as a prototype for studies of cytokine involvement in various human diseases.

摘要

非同位素原位杂交（NISH）是目前分子病理学中用于在新鲜和存档的人体活检组织中鉴定DNA的一种快速且灵敏的方法。在本研究中，我们将用于mRNA检测的NISH技术进行了扩展，包括使用地高辛标记的核糖探针来定位石蜡包埋材料（来自活动性炎症性肠病）中白细胞介素-4的低丰度mRNA。由于这种方法首次使对存档材料中细胞因子基因表达进行回顾性研究成为可能，它可作为研究细胞因子在各种人类疾病中作用的一个范例。