McGrory T, Meysick K, Lemchuk-Favel L T, Garber G E
Department of Microbiology & Immunology, University of Ottawa, Ontario, Canada.
J Parasitol. 1994 Feb;80(1):50-4.
Recent work with a mouse model of Trichomonas vaginalis infection indicated that estrogenized BALB/c mice that were preinfected with Lactobacillus acidophilus showed a greater duration of T. vaginalis infection as compared to a control group of mice that were not treated with L. acidophilus. To examine the interaction between T. vaginalis and L. acidophilus further we performed in vitro competitive growth assays between the 2 species. Although the addition of L. acidophilus to the T. vaginalis cultures slowed the growth of the protozoa, the added bacteria did not increase trichomonad death. However, T. vaginalis had a deleterious effect on L. acidophilus growth in combined cultures when compared to matched controls. Using an initial inoculum of 10(5)/ml, at 40 hr the control L. acidophilus concentrations had grown to 1.3 x 10(7)/ml. However, in combined culture with T. vaginalis, L. acidophilus concentrations at 40 hr had fallen to 7.8 x 10(5)/ml and 6.1 x 10(4)/ml for the 10:1 (T. vaginalis at 10(4)) and 1:1 (T. vaginalis at 10(5) test ratios, respectively (P < 0.01). This demonstrates that T. vaginalis can cause the concentration of L. acidophilus to fall in vitro and may explain why the concentration of L. acidophilus in the vagina falls in trichomoniasis.
最近对阴道毛滴虫感染小鼠模型的研究表明，与未用嗜酸乳杆菌处理的对照组小鼠相比，预先感染嗜酸乳杆菌的雌激素化BALB/c小鼠的阴道毛滴虫感染持续时间更长。为了进一步研究阴道毛滴虫与嗜酸乳杆菌之间的相互作用，我们对这两种菌进行了体外竞争性生长试验。虽然在阴道毛滴虫培养物中添加嗜酸乳杆菌减缓了原虫的生长，但添加的细菌并没有增加滴虫的死亡。然而，与匹配的对照组相比，在混合培养物中阴道毛滴虫对嗜酸乳杆菌的生长有有害影响。使用10(5)/ml的初始接种量，在40小时时，对照嗜酸乳杆菌浓度已增长至1.3×10(7)/ml。然而，在与阴道毛滴虫的混合培养中，对于10:1（阴道毛滴虫为10(4)）和1:1（阴道毛滴虫为10(5)）的试验比例，40小时时嗜酸乳杆菌浓度分别降至7.8×10(5)/ml和6.1×10(4)/ml（P<0.01）。这表明阴道毛滴虫可导致体外嗜酸乳杆菌浓度下降，这可能解释了为什么滴虫病患者阴道中嗜酸乳杆菌浓度会下降。
Zotero 插件