Bockisch A
Johannes Gutenberg-Universität, Mainz, Germany.
J Nucl Med. 1993 Jul;34(7):1134-9.
Dynamic in vivo imaging of spermatozoa radiolabeled with 99mTc-HMPAO in the female genital tract was performed noninvasively. Animal studies were performed under a variety of insemination conditions and their influence on the dynamics and number of spermatozoa in ascent was demonstrated. Spermatozoa motility was not significantly reduced by radiolabeling. In vitro and in vivo labeling stability was > 93% after 18 hr. Female rabbits were inseminated with labeled spermatozoa and dynamic scintigraphic studies were performed up to 12 hr after insemination. The in vivo behavior of labeled spermatozoa was similar to native spermatozoa. Absolute spermatozoa numbers could be determined noninvasively and reliably at different parts in the female genital tract at various timepoints after insemination.
对用99mTc-HMPAO放射性标记的精子在雌性生殖道内进行了无创动态体内成像。在多种授精条件下进行了动物研究，并证明了它们对精子上行动力学和数量的影响。放射性标记并未显著降低精子活力。18小时后体外和体内标记稳定性>93%。用标记的精子对雌性兔子进行授精，并在授精后长达12小时进行动态闪烁扫描研究。标记精子的体内行为与天然精子相似。授精后不同时间点可在雌性生殖道的不同部位无创且可靠地确定精子绝对数量。
