Kataoka H, DeCastro R, Zucker S, Biswas C
Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, Massachusetts 02111.
Cancer Res. 1993 Jul 1;53(13):3154-8.
The tumor cell-derived collagenase-stimulatory factor (TCSF) was previously purified from human lung carcinoma cells (S. M. Ellis, K. Nabeshima, and C. Biswas, Cancer Res., 49: 3385-3391, 1989). This protein is present on the surface of several types of human tumor cells in vitro and in vivo and stimulates production of interstitial collagenase in human fibroblasts. In this study it is shown that TCSF stimulates expression in human fibroblasts of mRNA for stromelysin 1 and 72-kDa gelatinase/type IV collagenase, as well as for interstitial collagenase. Measurement of enzyme protein by immunoassay showed that the amounts of interstitial collagenase and stromelysin 1 were increased in TCSF-treated fibroblasts; gelatin zymography indicated that there was an increase in the 72-kDa gelatinase. These results indicate that tumor cell interaction with fibroblasts via TCSF could lead to increased degradation of interstitial or basement membrane matrix components and thus to enhanced tumor cell invasion.
肿瘤细胞衍生的胶原酶刺激因子（TCSF）先前已从人肺癌细胞中纯化出来（S.M.埃利斯、K.锅岛和C.比斯瓦斯，《癌症研究》，49:3385 - 3391，1989年）。这种蛋白质在体外和体内的几种人类肿瘤细胞表面均有存在，并能刺激人成纤维细胞中间质胶原酶的产生。在本研究中发现，TCSF能刺激人成纤维细胞中基质溶解素1、72 kDa明胶酶/IV型胶原酶以及间质胶原酶的mRNA表达。通过免疫测定法对酶蛋白进行检测表明，经TCSF处理的成纤维细胞中间质胶原酶和基质溶解素1的量有所增加；明胶酶谱分析表明72 kDa明胶酶有所增加。这些结果表明，肿瘤细胞通过TCSF与成纤维细胞相互作用可能导致间质或基底膜基质成分的降解增加，从而增强肿瘤细胞的侵袭能力。
