嗜杀假丝酵母和树干毕赤酵母亲本菌株与融合菌株之间的基因组比较。

Genomic comparisons among parental and fusant strains of Candida shehatae and Pichia stipitis.

作者信息

Selebano E T, Govinden R, Pillay D, Pillay B, Gupthar A S

机构信息

Department of Biochemistry, University of the North, Sovenga, South Africa.

出版信息

Curr Genet. 1993 May-Jun;23(5-6):468-71. doi: 10.1007/BF00312636.

DOI:10.1007/BF00312636

PMID:8319304

Abstract

DNA-DNA binding experiments on selected fusants of Candida shehatae and Pichia stipitis showed that the nucleus of these strains was composed predominantly of Pichia DNA. Electrophoretic karyotyping revealed that the fusants contained four chromosomes, similar to those found in the Pichia parental strain. In addition, the fusants showed only marginal increases in cell DNA content when compared with the parents. Karyogamy was confirmed, however, by the isolation of recombinant phenotypic segregants, induced by meiotic and mitotic segregation. The results suggest that the fusion led to integration of Candida genes, rather than whole chromosomes, with the entire genome of P. stipitis.

摘要

对选定的嗜杀假丝酵母和树干毕赤酵母融合体进行的DNA-DNA结合实验表明，这些菌株的细胞核主要由毕赤酵母DNA组成。脉冲场凝胶电泳核型分析显示，融合体含有四条染色体，与毕赤酵母亲本菌株中的染色体相似。此外，与亲本相比，融合体的细胞DNA含量仅略有增加。然而，通过减数分裂和有丝分裂分离诱导的重组表型分离株的分离证实了核融合。结果表明，融合导致嗜杀假丝酵母基因与树干毕赤酵母的整个基因组整合，而不是整条染色体。