Dawra R, Saluja A, Lerch M M, Saluja M, Logsdon C, Steer M
Department of Surgery, Beth Israel Hospital/Harvard Medical School, Boston, MA 02215.
Biochem Biophys Res Commun. 1993 Jun 30;193(3):814-20. doi: 10.1006/bbrc.1993.1698.
Pancreatic acinar cells possess both high low affinity receptors for cholecystokinin. The cholecystokinin analog caerulein, which exerts a trophic effect on the rat pancreas, acts as an agonist at both types of receptors. In contrast, the synthetic analog CCK-JMV-180, which also acts as an agonist at high affinity receptors, opposes the action of caerulein on the low affinity receptors. We report that infusion of either caerulein or CCK-JMV-180 into rats increases [3H]-thymidine incorporation into pancreatic DNA and causes the pancreatic weight as well as content of DNA, RNA, and protein to increase. CCK-JMV-180 also stimulates in-vitro incorporation of [3H]-thymidine into DNA of cultured rat acini. The finding that both caerulein and CCK-JMV-180 exert the same trophic effect on pancreatic acinar cells indicates that this effect is mediated via high affinity acinar cell cholecystokinin receptors.
胰腺腺泡细胞拥有胆囊收缩素的高亲和力和低亲和力受体。胆囊收缩素类似物蛙皮素对大鼠胰腺具有营养作用，它在这两种受体上均作为激动剂发挥作用。相比之下，合成类似物CCK-JMV-180同样在高亲和力受体上作为激动剂起作用，却拮抗蛙皮素对低亲和力受体的作用。我们报告称，向大鼠体内输注蛙皮素或CCK-JMV-180会增加[3H] - 胸腺嘧啶核苷掺入胰腺DNA的量，并导致胰腺重量以及DNA、RNA和蛋白质的含量增加。CCK-JMV-180还能刺激体外培养的大鼠腺泡中[3H] - 胸腺嘧啶核苷掺入DNA。蛙皮素和CCK-JMV-180对胰腺腺泡细胞均发挥相同营养作用这一发现表明，该作用是通过腺泡细胞高亲和力胆囊收缩素受体介导的。
