转录因子TFIIB位点对于与启动子结合的TFIID相互作用很重要。

Transcription factor TFIIB sites important for interaction with promoter-bound TFIID.

作者信息

Yamashita S, Hisatake K, Kokubo T, Doi K, Roeder R G, Horikoshi M, Nakatani Y

机构信息

National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, MD 20892.

出版信息

Science. 1993 Jul 23;261(5120):463-6. doi: 10.1126/science.8332911.

DOI:10.1126/science.8332911

PMID:8332911

Abstract

Transcription initiation factor TFIIB recruits RNA polymerase II to the promoter subsequent to interaction with a preformed TFIID-promoter complex. The domains of TFIIB required for binding to the TFIID-promoter complex and for transcription initiation have been determined. The carboxyl-terminal two-thirds of TFIIB, which contains two direct repeats and two basic residue repeats, is sufficient for interaction with the TFIID-promoter complex. An extra 84-residue amino-terminal region, with no obvious known structural motifs, is required for basal transcription activity. Basic residues within the second basic repeat of TFIIB are necessary for stable interaction with the TFIID-promoter complex, whereas the basic character of the first basic repeat is not. Functional roles of other potential structural motifs are discussed in light of the present study.

摘要

转录起始因子TFIIB在与预先形成的TFIID-启动子复合物相互作用后，将RNA聚合酶II招募到启动子上。已确定了TFIIB与TFIID-启动子复合物结合以及转录起始所需的结构域。TFIIB的羧基末端三分之二包含两个直接重复序列和两个碱性残基重复序列，足以与TFIID-启动子复合物相互作用。基础转录活性需要一个额外的84个残基的氨基末端区域，该区域没有明显已知的结构基序。TFIIB第二个碱性重复序列中的碱性残基对于与TFIID-启动子复合物的稳定相互作用是必需的，而第一个碱性重复序列的碱性特征则不是。根据本研究讨论了其他潜在结构基序的功能作用。