Alderfer J L, Soni S D, Arakali A V, Wallace J C
Biophysics Department, Roswell Park Cancer Institute, Buffalo, NY 14263.
Photochem Photobiol. 1993 May;57(5):770-6. doi: 10.1111/j.1751-1097.1993.tb09209.x.
The acetone-sensitized irradiation using UV-B (ultraviolet light, 280-320 nm; sunlamps) of thymidylyl(3'-->5')deoxyfluorouridine monophosphate produces two main photoproducts. The distribution of these photoproducts is dependent on the pH of the irradiation solution. At pH 6, the cis-syn cyclobutane-type photodimer is the major product, whereas at high pH (8-10) a photoadduct is the major product. These photoproducts have been identified and structurally characterized by H-1 and C-13 NMR spectroscopy. The photoadduct arises from defluorination of the 5-fluorouracil moiety. The structure of the photoadduct maintains the sugar-phosphate backbone of the starting material (d-TpF), and contains a saturated thymine moiety with an added Thy(C6-hydroxyl) and a Thy(C5)-(C5)Ura covalent bond.
使用紫外线B（紫外线，280 - 320纳米；太阳灯）对胸苷酰（3'→5'）脱氧氟尿苷单磷酸进行丙酮敏化照射会产生两种主要的光产物。这些光产物的分布取决于照射溶液的pH值。在pH 6时，顺式-顺环丁烷型光二聚体是主要产物，而在高pH值（8 - 10）时，光加合物是主要产物。这些光产物已通过氢-1和碳-13核磁共振光谱进行了鉴定和结构表征。光加合物源自5-氟尿嘧啶部分的脱氟反应。光加合物的结构保留了起始材料（d-TpF）的糖-磷酸主链，并包含一个饱和胸腺嘧啶部分，带有一个额外的Thy（C6-羟基）和一个Thy（C5）-（C5）尿嘧啶共价键。
