Ni Z, Li S, Liu Y, Tang Y, Pang D
Hua Xi Yi Ke Da Xue Xue Bao. 1993 Mar;24(1):82-6.
A total of 22 organophosphorus pesticides (OPPs), including 8 ethyl-, 9 methyl-, and 5 other OPPs, were tested for mutagenicity in micronucleus assay system both in 615 mouse marrow cells in vivo with multi-intraperitoneal administrations and in cultured Chinese hamster lung (CHL) cells in vitro; and structure-mutagenicity relationship of OPPs was analyzed. Among the OPPs tested in vivo, 5 ethyl-(diazinon, chlorpyrifos, disulfoton, ethion, and parathion), and only 1 methyl-(dimethoate) were found mutagenic, while the other OPPs were negative. Six ethyl- (azinphos ethyl, chlorpyrifos, ethion, parathion, phosaione, and quinaphos), 8 methyl- (azinophos methyl, chlorpyrifos methyl, dichlorvos, dimethoate, fenitrothion, malathion, parathion methyl, and trimethyl phosphate), and 2 other OPPs (EII and MIA), however, induced micronucleus in CHL cells in vitro. The results indicated that most of the ethyl-OPPs tested showed the ability to induce micronucleus both in vivo and in vitro, and that most of the methyl-OPPs were positive only in vitro. The mechanism for the adversity of mutagenic activity in micronucleus assay in vivo and in vitro produced by different kinds of OPPs was also discussed.
总共22种有机磷农药（OPPs），包括8种乙基、9种甲基和5种其他有机磷农药，在体内通过多次腹腔注射对615小鼠骨髓细胞以及在体外对培养的中国仓鼠肺（CHL）细胞进行微核试验系统的致突变性测试；并分析了有机磷农药的结构-致突变性关系。在体内测试的有机磷农药中，发现5种乙基（二嗪农、毒死蜱、乙拌磷、乙硫磷和对硫磷）以及仅1种甲基（乐果）具有致突变性，而其他有机磷农药为阴性。然而，6种乙基（乙基谷硫磷、毒死蜱、乙硫磷、对硫磷、亚胺硫磷和喹硫磷）、8种甲基（甲基谷硫磷、甲基毒死蜱、敌敌畏、乐果、杀螟硫磷、马拉硫磷、甲基对硫磷和磷酸三甲酯）以及2种其他有机磷农药（EII和MIA）在体外CHL细胞中诱导产生微核。结果表明，大多数测试的乙基有机磷农药在体内和体外均显示出诱导微核的能力，而大多数甲基有机磷农药仅在体外呈阳性。还讨论了不同种类有机磷农药在体内和体外微核试验中致突变活性差异的机制。
