一种对人白细胞介素-2受体具有特异性的鼠/人嵌合抗体的开发及功能特性研究

Development and functional characterization of a murine/human chimeric antibody with specificity for the human interleukin-2 receptor.

作者信息

Vandevyver C, Steukers M, Lambrechts J, Heyligen H, Raus J

机构信息

Dr L. Willems-Instituut, Universitaire Campus, Diepenbeek, Belgium.

出版信息

Mol Immunol. 1993 Jul;30(10):865-76. doi: 10.1016/0161-5890(93)90010-9.

DOI:10.1016/0161-5890(93)90010-9

PMID:8341281

Abstract

A murine/human chimeric antibody, with specificity for the human interleukin-2 receptor, was developed by genetic engineering. For this purpose, the light and heavy chain variable region exons encoding the murine monoclonal antibody 2C8 were isolated and inserted into expression vectors containing the human kappa and gamma-1 constant regions. After transfection by electroporation of the chimeric genes into murine Sp 2/0 hybridoma cells, transfectomas secreting the complete chimeric antibody were selected. The chimeric antibody has similar binding properties as the original murine antibody. The in vitro cytotoxic effects of the murine and the chimeric antibodies were compared.

摘要

通过基因工程开发了一种对人白细胞介素-2受体具有特异性的鼠/人嵌合抗体。为此，分离出编码鼠单克隆抗体2C8的轻链和重链可变区外显子，并将其插入含有人类κ和γ-1恒定区的表达载体中。通过电穿孔将嵌合基因转染到鼠Sp 2/0杂交瘤细胞中后，筛选出分泌完整嵌合抗体的转染瘤细胞。该嵌合抗体具有与原始鼠抗体相似的结合特性。比较了鼠抗体和嵌合抗体的体外细胞毒性作用。