锂对溴脱氧尿苷和氚标记胸腺嘧啶核苷掺入人甲状旁腺细胞的影响。

Effect of lithium on incorporation of bromodeoxyuridine and tritiated thymidine into human parathyroid cells.

作者信息

Saxe A, Gibson G

机构信息

Department of Surgery, Sinai Hospital, Detroit, Mich.

出版信息

Arch Surg. 1993 Aug;128(8):865-9. doi: 10.1001/archsurg.1993.01420200039007.

DOI:10.1001/archsurg.1993.01420200039007

PMID:8343059

Abstract

OBJECTIVE

To measure lithium chloride-stimulated incorporation of tritiated thymidine (3H-TdR) and bromodeoxyuridine (BrdU) by human parathyroid cells as an indicator of lithium's growth-promoting properties.

DESIGN

Cell suspensions derived from abnormal human parathyroid tissue were incubated for 5 days with graded concentrations of lithium and pulsed with either 0.074 MBq of 3H-TdR or 0.6 microgram of BrdU. The 3H-TdR incorporation was measured by counting cell filtrates in a beta counter, and nuclear BrdU incorporation was determined by counting nuclei positively stained by an antibody to BrdU. Counts of BrdU-positive cells by two observers unaware of incubation conditions were correlated with 3H-TdR counts.

RESULTS

Cell suspensions derived from adenomatous and hyperplastic parathyroid glands demonstrated a dose-related increase in 3H-TdR incorporation. There was close correlation between the two observers' counts of BrdU-positive cells (r = 0.70, P = .01) and between each observer's counts and 3H-TdR counts (r = 0.70, P = .02, and r = 0.90, P = .01, respectively).

CONCLUSIONS

Lithium stimulates in vitro incorporation of 3H-TdR and BrdU by abnormal human parathyroid cells, consistent with a growth-promoting effect.

摘要

目的

检测氯化锂刺激人甲状旁腺细胞掺入氚标记胸腺嘧啶核苷（³H-TdR）和溴脱氧尿苷（BrdU）的情况，以此作为锂具有促生长特性的指标。

设计

将来源于异常人甲状旁腺组织的细胞悬液与不同浓度的锂孵育5天，并用0.074MBq的³H-TdR或0.6微克的BrdU进行脉冲标记。通过在β计数器中计数细胞滤液来测量³H-TdR掺入量，通过计数被抗BrdU抗体阳性染色的细胞核来测定细胞核BrdU掺入量。由两名不知孵育条件的观察者对BrdU阳性细胞进行计数，并与³H-TdR计数结果进行相关性分析。

结果

来源于腺瘤性和增生性甲状旁腺的细胞悬液显示³H-TdR掺入量呈剂量依赖性增加。两名观察者对BrdU阳性细胞的计数之间存在密切相关性（r = 0.70，P = 0.01），且每名观察者的计数与³H-TdR计数之间也存在密切相关性（分别为r = 0.70，P = 0.02和r = 0.90，P = 0.01）。