Flieger K, Oelmüller R, Herrmann R G
Botanisches Institut, Ludwig-Maximilians-Universität, München, FRG.
Plant Mol Biol. 1993 Jul;22(4):703-9. doi: 10.1007/BF00047411.
Several cDNA clones encoding subunit XI of photosystem I reaction center (PSI-L) have been isolated from two lambda gt11 expression libraries based on polyadenylated RNA of spinach seedlings illuminated for 4 and 16 h, respectively. The precursor polypeptide made from these recombinant DNAs in vitro can be efficiently imported into isolated spinach chloroplasts. It is correctly processed to the size of the authentic polypeptide and integrates into the photosystem I assembly. The 834 nucleotide sequence of the longest cDNA insert encodes a precursor polypeptide of 24 kDa (216 residues) and a mature protein of probably 18.8 kDa (169 residues). Hydropathy analysis suggests that the polypeptide contains two transmembrane segments. The protein appears to originate in a single-copy gene in spinach and to be decoded from RNA species of ca. 900 bases.
已经分别从两个基于菠菜幼苗经4小时和16小时光照后的聚腺苷酸化RNA构建的λgt11表达文库中分离出了几个编码光系统I反应中心亚基XI（PSI-L）的cDNA克隆。由这些重组DNA在体外产生的前体多肽能够有效地导入分离的菠菜叶绿体中。它被正确加工成与天然多肽相同的大小，并整合到光系统I组件中。最长cDNA插入片段的834个核苷酸序列编码一个24 kDa（216个氨基酸残基）的前体多肽和一个可能为18.8 kDa（169个氨基酸残基）的成熟蛋白。亲水性分析表明该多肽含有两个跨膜区段。该蛋白似乎起源于菠菜中的一个单拷贝基因，并由约900个碱基的RNA种类解码而来。
