五氯苯酚对人红细胞乙酰胆碱酯酶的高度协同抑制作用。
Highly cooperative inhibition of acetylcholinesterase by pentachlorophenol in human erythrocytes.
作者信息
Igisu H, Hamasaki N, Ikeda M
机构信息
Department of Environmental Toxicology, University of Occupational and Environmental Health, Kitakyushu, Japan.
出版信息
Biochem Pharmacol. 1993 Jul 6;46(1):175-7. doi: 10.1016/0006-2952(93)90362-z.
Pentachlorophenol (PCP) inhibited acetylcholinesterase (AchE) activity in human erythrocyte membranes with high cooperativity. The Hill coefficient for the inhibition was 4-5 in "untreated" membranes. Differences in the temperature (13, 25 and 37 degrees) or treatment with 1% Triton X-100 did not clearly affect the cooperativity which, however, increased after the erythrocyte membranes were treated with 2-mercaptoethanol and iodoacetatic acid, suggesting that higher cooperativity in the inhibition of AchE by PCP may reflect conformational changes of AchE. Thus, PCP may be useful for the study of AchE in human erythrocytes.
五氯苯酚（PCP）以高协同性抑制人红细胞膜中的乙酰胆碱酯酶（AchE）活性。在“未处理”的膜中，抑制作用的希尔系数为4至5。温度（13、25和37摄氏度）的差异或用1% Triton X - 100处理并未明显影响协同性，然而，在用2 - 巯基乙醇和碘乙酸处理红细胞膜后，协同性增加，这表明PCP对AchE抑制作用中更高的协同性可能反映了AchE的构象变化。因此，PCP可能有助于研究人红细胞中的AchE。
Zotero 插件