Merchant T E, de Graaf P W, Minsky B D, Obertop H, Glonek T
Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
NMR Biomed. 1993 May-Jun;6(3):187-93. doi: 10.1002/nbm.1940060304.
Phospholipid extracts of surgical tissue specimens from 18 patients, consisting of normal esophagus, distal esophageal tumor and normal stomach, were analyzed using 31P NMR. The prominent phospholipids detected in these tissues included cardiolipin (CL), phosphatidylethanolamine plasmalogen, phosphatidylethanolamine (PE), phosphatidylserine (PS), sphingomyelin (SPH), phosphatidylinositol (PI), phosphatidylcholine plasmalogen and phosphatidylcholine (PC). Very small quantities of the phospholipids lysophosphatidylcholine, phosphatidic acid, phosphatidylglycerol, and an uncharacterized phospholipid at -0.13 delta also were detected in some of the 54 tissue specimens analyzed. The mean relative concentrations of these phospholipids, in mole percentages of total detected phosphorus, were determined from the acquired spectra and used to differentiate among the three tissue groups. The relative concentrations of the following phospholipids differed significantly (p < 0.001) among the respective tissue groups: normal esophagus vs esophageal tumor, PS, SPH, PI, PC; normal esophagus vs normal stomach, CL, PE, PS, SPH; esophageal tumor vs normal stomach, CL, PE. Membrane phospholipids implicated in modulating the growth and metastases of tumors of epithelial origin can be profiled to discriminate among normal esophagus, distal esophageal tumor and normal stomach using 31P NMR.
使用31P NMR分析了18例患者手术组织标本的磷脂提取物，这些标本包括正常食管、食管远端肿瘤和正常胃。在这些组织中检测到的主要磷脂包括心磷脂（CL）、磷脂酰乙醇胺缩醛磷脂、磷脂酰乙醇胺（PE）、磷脂酰丝氨酸（PS）、鞘磷脂（SPH）、磷脂酰肌醇（PI）、磷脂酰胆碱缩醛磷脂和磷脂酰胆碱（PC）。在分析的54个组织标本中的一些标本中还检测到了极少量的磷脂溶血磷脂酰胆碱、磷脂酸、磷脂酰甘油以及化学位移为-0.13处一种未鉴定的磷脂。根据获得的光谱确定这些磷脂的平均相对浓度，以总检测磷的摩尔百分比表示，并用于区分这三个组织组。以下磷脂在各自组织组之间的相对浓度存在显著差异（p<0.001）：正常食管与食管肿瘤相比，PS、SPH、PI、PC；正常食管与正常胃相比，CL、PE、PS、SPH；食管肿瘤与正常胃相比，CL、PE。利用31P NMR对与调节上皮源性肿瘤生长和转移有关的膜磷脂进行分析，可区分正常食管、食管远端肿瘤和正常胃。
