Dubus I, Rappaport L, Barrieux A, Lompré A M, Schwartz K, Samuel J L
Unité 127 INSERM, Hôpital Lariboisière, Paris, France.
Pflugers Arch. 1993 Jun;423(5-6):455-61. doi: 10.1007/BF00374941.
The effects of two adhesion substrates (serum and laminin) and time in culture on the expression of genes encoding myosin heavy chain (MHC) isoforms and alpha-skeletal actin were analysed in myocytes isolated from adult rat heart and maintained in serum-free culture. Relative messenger ribonucleic acid (mRNA) abundances were quantitated by dot-blot analysis. Gene expression was not influenced by the substrate used. Time in culture induced a decrease in total mRNA abundance and an up-regulation of beta-MHC and alpha-skeletal actin genes. It is proposed that atrophy of adult myocytes is associated with a pattern of gene expression similar to the fetal program.
在从成年大鼠心脏分离并维持在无血清培养条件下的心肌细胞中，分析了两种黏附底物（血清和层粘连蛋白）以及培养时间对编码肌球蛋白重链（MHC）亚型和α-骨骼肌肌动蛋白的基因表达的影响。通过斑点印迹分析对相对信使核糖核酸（mRNA）丰度进行定量。基因表达不受所用底物的影响。培养时间导致总mRNA丰度下降以及β-MHC和α-骨骼肌肌动蛋白基因上调。有人提出，成年心肌细胞萎缩与类似于胎儿程序的基因表达模式有关。
