Pourshadi M, De Fazio S R, Gozzo J J
Department of Medical Laboratory Science, Bouvé College of Pharmacy and Health Sciences, Northeastern University, Boston, Massachusetts 02115.
Transplantation. 1993 Aug;56(2):385-90. doi: 10.1097/00007890-199308000-00026.
To investigate whether a veto cell phenomenon is involved in the ability of donor strain bone marrow cells (BMC) to inactivate second-set skin graft rejection induced by the adoptive transfer of sensitized spleen cells (SSC), mice were grafted with ear skin allografts and injected on the following day with recipient strain SSC or SSC plus donor strain BMC. When skin and BMC donors from an F1 strain (B6AF1) were combined with a parental strain (C57BL/6) as recipient and SSC donors, the BMC were fully active in abrogating second-set rejection even though the SSC were naturally tolerant of the BMC. Thus, immunological recognition of the SSC by the BMC is not necessary for this abrogation. The suppressive activity of the BMC is specific in a manner similar to that reported for veto cells (i.e., they suppress an anti-self response). If BMC from various strains of mice were used to alter the rejection of C3H grafts by B6AF1 recipients, only BMC expressing the MHC antigens to which the SSC had been sensitized significantly suppressed the activity of SSC. Sharing of additional antigens between the graft and BMC (e.g., MHC antigens not recognized by the SSC or minor antigens) was neither necessary nor sufficient. Mitomycin C-treated BMC were unable to inhibit SSC-induced accelerated rejection. Overall, the characteristics of the SSC-suppressive activity of BMC are consistent with a veto cell nature of the BMC.
为了研究否决细胞现象是否参与供体品系骨髓细胞（BMC）灭活由致敏脾细胞（SSC）过继转移诱导的二次皮肤移植排斥反应的能力，将小鼠进行耳部皮肤同种异体移植，并在第二天注射受体品系SSC或SSC加供体品系BMC。当来自F1品系（B6AF1）的皮肤和BMC供体与作为受体和SSC供体的亲本品系（C57BL/6）组合时，即使SSC对BMC天然耐受，BMC在废除二次排斥反应中仍完全有效。因此，BMC对SSC的免疫识别对于这种废除不是必需的。BMC的抑制活性具有特异性，其方式类似于报道的否决细胞（即，它们抑制抗自身反应）。如果使用来自各种小鼠品系的BMC来改变B6AF1受体对C3H移植的排斥反应，只有表达SSC已致敏的MHC抗原的BMC才能显著抑制SSC的活性。移植和BMC之间共享额外的抗原（例如，SSC未识别的MHC抗原或次要抗原）既不是必需的也不是充分的。丝裂霉素C处理的BMC无法抑制SSC诱导的加速排斥反应。总体而言，BMC对SSC的抑制活性特征与BMC的否决细胞性质一致。
