IV型胶原、层粘连蛋白和纤连蛋白可促进兔晶状体上皮细胞在体外的黏附和迁移。

Type IV collagen, laminin, and fibronectin promote the adhesion and migration of rabbit lens epithelial cells in vitro.

作者信息

Olivero D K, Furcht L T

机构信息

Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis 55455.

出版信息

Invest Ophthalmol Vis Sci. 1993 Sep;34(10):2825-34.

PMID:8360016

Abstract

PURPOSE

To assess the ability of basement membrane and extracellular matrix proteins to promote rabbit lens epithelial cell adhesion and migration, which may play a role in the development of secondary cataract.

METHODS

Rabbit lens epithelial cells were isolated and grown in tissue culture for use in standardized assays to study adhesion and migration of rabbit lens epithelial cells in response to type IV collagen, laminin, fibronectin, and ovalbumin.

RESULTS

Under these conditions, the adhesion of rabbit lens epithelial cells to surfaces coated with varying concentrations of type IV collagen, laminin, and fibronectin was shown to be dependent on concentration. Rabbit lens epithelial cells did not adhere to ovalbumin-coated surfaces at any concentration tested. Type IV collagen promotes maximal in vitro adhesion of rabbit lens epithelial cells at lower coating concentrations in comparison to laminin and fibronectin. In cell migration experiments, fibronectin promoted maximal migration at lower concentrations in comparison with laminin and type IV collagen. This was shown both in haptotaxis experiments (the migration of cells to surfaces coated with protein) and in chemotaxis experiments (the migration of cells to attractants in solution). Lens epithelial cells did not migrate in response to ovalbumin under the conditions of this study.

CONCLUSION

The results of these studies indicate that adhesion and migration of lens epithelial cells occurs in response to the lens capsule proteins type IV collagen and laminin and in response to fibronectin, a protein found in the lens during embryologic development. Because fibronectin plays a role in the embryologic development of the lens but is not normally present in the adult lens, the possible introduction of fibronectin into the eye after surgery may play a critical role in the posterior migration of lens epithelial cells and the development of posterior capsular opacification or secondary cataract.

摘要

目的

评估基底膜和细胞外基质蛋白促进兔晶状体上皮细胞黏附和迁移的能力，这可能在继发性白内障的发生发展中起作用。

方法

分离兔晶状体上皮细胞并在组织培养中生长，用于标准化试验，以研究兔晶状体上皮细胞对IV型胶原、层粘连蛋白、纤连蛋白和卵清蛋白的黏附和迁移反应。

结果

在这些条件下，兔晶状体上皮细胞对涂有不同浓度IV型胶原、层粘连蛋白和纤连蛋白的表面的黏附显示出浓度依赖性。在任何测试浓度下，兔晶状体上皮细胞都不黏附于涂有卵清蛋白的表面。与层粘连蛋白和纤连蛋白相比，IV型胶原在较低包被浓度下促进兔晶状体上皮细胞的最大体外黏附。在细胞迁移实验中，与层粘连蛋白和IV型胶原相比，纤连蛋白在较低浓度下促进最大迁移。这在趋触性实验（细胞向涂有蛋白质的表面迁移）和趋化性实验（细胞向溶液中的吸引剂迁移）中均得到证实。在本研究条件下，晶状体上皮细胞对卵清蛋白无迁移反应。

结论

这些研究结果表明，晶状体上皮细胞的黏附和迁移是对晶状体囊膜蛋白IV型胶原和层粘连蛋白以及纤连蛋白（一种在胚胎发育期间在晶状体中发现的蛋白质）的反应。由于纤连蛋白在晶状体的胚胎发育中起作用，但在成年晶状体中通常不存在，因此手术后纤连蛋白可能引入眼内，这可能在晶状体上皮细胞的后向迁移以及后囊膜混浊或继发性白内障的发生发展中起关键作用。