Suzuki T, Amano K, Kuroso K, Fukutake K, Fujimaki M
Department of Clinical Pathology, Tokyo Medical College.
Rinsho Byori. 1993 Feb;41(2):215-9.
Prothrombin fragment F1.2 (F1.2) is a new molecular marker indicating acceleration of blood coagulation. We evaluated a new assay of F1.2 measurement using a micro-titer plate (Dade Prothrombin Fragment F1.2 ELISA: Baxter Diagnostics Inc., U.S.A.). The assay obtained satisfactory results in intra-assay reproducibility test, inter-assay reproducibility test, dilution linearity test and in vitro recovery test. Normal values of plasma F1.2 were 0.16 +/- 0.09 nmol/l (mean +/- SD) in 108 healthy individuals. Differences in the levels between the sexes were not significant. In patients with DIC (n = 22), plasma F1.2 levels were significantly higher than in normal healthy individuals and were correlated with the levels of thrombin-antithrombin III complex. These findings suggest that this F1.2 assay using a micro-titer plate is clinically useful for the evaluation of the therapeutic effect and diagnosis of hypercoagulable states like DIC.
凝血酶原片段F1.2（F1.2）是一种指示凝血加速的新分子标志物。我们评估了一种使用微量滴定板测量F1.2的新检测方法（达德凝血酶原片段F1.2酶联免疫吸附测定法：美国百特诊断公司）。该检测方法在批内重复性试验、批间重复性试验、稀释线性试验和体外回收率试验中均获得了满意的结果。108名健康个体的血浆F1.2正常值为0.16±0.09 nmol/l（平均值±标准差）。性别之间的水平差异不显著。在22例弥散性血管内凝血（DIC）患者中，血浆F1.2水平显著高于正常健康个体，且与凝血酶 - 抗凝血酶III复合物水平相关。这些发现表明，这种使用微量滴定板的F1.2检测方法在临床上对于评估治疗效果和诊断如DIC等高凝状态是有用的。
