Acosta G B, Otero Losada M E, Rubio M C
Instituto de Investigaciones Farmacológicas [ININFA], Consejo Nacional de Investigaciones Cientificas y Técnicas [CONICET], Buenos Aires, Argentina.
Neurosci Lett. 1993 May 14;154(1-2):175-8. doi: 10.1016/0304-3940(93)90200-5.
(1) The function of the gamma-aminobutyric acid (GABA)ergic system in certain areas of the rat brain was investigated after acute and chronic cold stress. (2) GABA concentration, [3H]GABA uptake and the activity of the synthesis enzyme glutamate decarboxylase (GAD) were measured. (3) Acute stress: (a) reduced GABA concentration in the corpus striatum (29%); (b) decreased GAD activity (under non-saturating substrate concentration) in the olfactory bulbs (24%); (c) diminished neuronal uptake of [3H]GABA in the frontal cerebral cortex (65%), hypothalamus (86%) and olfactory bulbs (82%). (4) Chronic stress: (a) reduced the endogenous levels of GABA in the frontal cerebral cortex (51%), hypothalamus (26%) and olfactory bulbs (15%); (b) decreased GAD activity in the corpus striatum (32%) and olfactory bulbs (34%); (c) decreased neuronal uptake of [3H]GABA in the hypothalamus (83%). (5) These findings suggest that compensatory changes may develop in the GABAergic system after chronic stress.
(1) 在急性和慢性冷应激后，对大鼠大脑某些区域的γ-氨基丁酸（GABA）能系统功能进行了研究。(2) 测量了GABA浓度、[3H]GABA摄取以及合成酶谷氨酸脱羧酶（GAD）的活性。(3) 急性应激：(a) 使纹状体中的GABA浓度降低（29%）；(b) 使嗅球中GAD的活性降低（在非饱和底物浓度下）（24%）；(c) 使额叶大脑皮质（65%）、下丘脑（86%）和嗅球（82%）中神经元对[3H]GABA的摄取减少。(4) 慢性应激：(a) 使额叶大脑皮质（51%）、下丘脑（26%）和嗅球（15%）中GABA的内源性水平降低；(b) 使纹状体（32%）和嗅球（34%）中GAD的活性降低；(c) 使下丘脑中神经元对[3H]GABA的摄取减少（83%）。(5) 这些发现表明，慢性应激后GABA能系统可能会发生代偿性变化。
