Kuji N, Sakaida M, Miyazaki T, Katayama E, Sugiyama T, Iida E
Department of Obstetrics and Gynecology, Ogikubo Hospital, Tokyo.
Nihon Sanka Fujinka Gakkai Zasshi. 1993 Sep;45(9):1001-6.
176 supernumerary human embryos following treatment by IVF were cryopreserved with dimethylsulfoxide (1.5M) and sucrose (0.1M) as cryoprotectants. The embryos were frozen at the early cleavage stage (48-96 hours after insemination) in the programmable freezer, by means of the protocol providing for slow cooling until -40 degrees C and slow thawing. The survival rate (50% or more blastomeres were intact) after thawing was higher for the embryos at the 8 cell stage to the morulla stage (60-96 hours after insemination) than for the embryos at the 4 to 6 cell stage (48-60 hours after insemination) (87% and 66%, p < 0.001), and the overall survival rate was 80% (140/176). Out of 62 transfer cycles, 11 pregnancies (17.7%) and 6 deliveries (9.7%) were achieved. No fetal malformation or chromosomal aberration was observed.
176 个经体外受精治疗后的多余人类胚胎，以二甲亚砜（1.5M）和蔗糖（0.1M）作为冷冻保护剂进行冷冻保存。胚胎在早期卵裂阶段（授精后 48 - 96 小时），通过程序降温冷冻仪，按照先缓慢降温至 -40℃再缓慢解冻的方案进行冷冻。解冻后的存活率（50%或更多卵裂球完整）在 8 细胞期至桑葚胚期（授精后 60 - 96 小时）的胚胎高于 4 至 6 细胞期（授精后 48 - 60 小时）的胚胎（分别为 87%和 66%，p < 0.001），总体存活率为 80%（140/176）。在 62 次移植周期中，实现了 11 例妊娠（17.7%）和 6 例分娩（9.7%）。未观察到胎儿畸形或染色体畸变。
