编码雄激素依赖性小鼠精囊分泌蛋白的cDNA的克隆与序列分析

Cloning and sequence analysis of a cDNA encoding an androgen-dependent mouse seminal vesicle secretory protein.

作者信息

Guilbaud C, Simon A M, Veyssière G, Jean C

机构信息

Physiologie Comparée et Endocrinologie, CNRS URA 360, Université Blaise Pascal, Aubière, France.

出版信息

J Mol Endocrinol. 1993 Jun;10(3):279-88. doi: 10.1677/jme.0.0100279.

DOI:10.1677/jme.0.0100279

PMID:8373512

Abstract

We report the cloning and sequencing of a new cDNA sequence encoding a protein from the mouse seminal vesicle. An open reading frame of 297 nucleotides encoded a protein of 99 amino acids with a calculated molecular mass of 11.454 kDa. The first 21 amino acids constituted a signal peptide followed by 78 amino acids encoding the secreted protein. The cDNA sequence comprised a 3' untranslated region of 226 bp and the polyadenylation signal AATAAA, 19 bp upstream from the poly(A)+ tail. A high degree of homology was found between this protein and members of the family of seminal vesicle secretory (SVS) proteins, especially rat SVS VI. Northern blot analysis indicated the presence of a 0.7 kb mRNA species in the mRNAs of seminal vesicle tissue. Castration resulted in a marked decrease in the level of the 0.7 kb mRNA encoding the protein, whereas administration of testosterone to castrated males restored the 0.7 kb mRNA.

摘要

我们报道了从小鼠精囊中克隆并测序的一种编码蛋白质的新cDNA序列。一个297个核苷酸的开放阅读框编码了一个99个氨基酸的蛋白质，计算分子量为11.454 kDa。前21个氨基酸构成一个信号肽，随后是78个编码分泌蛋白的氨基酸。该cDNA序列包括一个226 bp的3'非翻译区和聚腺苷酸化信号AATAAA，位于聚(A)+尾上游19 bp处。在该蛋白质与精囊分泌（SVS）蛋白家族成员之间发现了高度同源性，尤其是大鼠SVS VI。Northern印迹分析表明在精囊组织的mRNA中存在一种0.7 kb的mRNA种类。去势导致编码该蛋白质的0.7 kb mRNA水平显著下降，而对去势雄性小鼠给予睾酮可恢复0.7 kb mRNA。