Kotalová R, Lojda Z, Smídová J, Jodl J
I. dĕtská klinika 2. LF UK a FN v Motole, Praha.
Cesk Pediatr. 1993 Jul;48(7):398-403.
In the jejunal mucosae of 45 children with coeliac sprue (24 cases in the florid stage and 21 cases in remission) and 19 children without signs of affection of the small intestine a quantitative investigation was made of cells in the lamina propria which were visualized selectively by histochemical and immunohistochemical methods-plasmocytes with IgA, plasmocytes with IgM, plasmocytes with IgG, cells containing IgE, mast cells, eosinophil and neutrophil leucocytes. The objective was to find which of the above cells will best reveal by a change of their number the pathological process in the lamina propria of children with coeliac sprue and which has the greatest informative value on the present state of the lamina propria. It was found that plasmocytes with IgG and cells containing IgE are good markers of the activity of coeliac sprue in the lamina propria. The best marker of the activity of coeliac sprue in the lamina propria are mast cells detected by the histochemical reaction for tryptase using Z-Ala-Ala-Lys-MNA. This reaction detects mast cells also in non-fixed bioptic material.
对45例乳糜泻患儿（24例处于急性期，21例处于缓解期）和19例无小肠病变迹象的儿童的空肠黏膜进行了研究，采用组织化学和免疫组织化学方法对固有层细胞进行定量分析，这些细胞包括含IgA的浆细胞、含IgM的浆细胞、含IgG的浆细胞、含IgE的细胞、肥大细胞、嗜酸性粒细胞和中性粒细胞。目的是找出上述哪些细胞通过数量变化能最有效地揭示乳糜泻患儿固有层的病理过程，以及哪些细胞对固有层的当前状态具有最大的信息价值。研究发现，含IgG的浆细胞和含IgE的细胞是固有层乳糜泻活动的良好标志物。使用Z - Ala - Ala - Lys - MNA通过对色氨酸酶的组织化学反应检测到的肥大细胞是固有层乳糜泻活动的最佳标志物。该反应也能在未固定的活检材料中检测到肥大细胞。
