人牛磺酸转运体的克隆及甲状腺细胞中牛磺酸摄取的特性研究。
Cloning of the human taurine transporter and characterization of taurine uptake in thyroid cells.
作者信息
Jhiang S M, Fithian L, Smanik P, McGill J, Tong Q, Mazzaferri E L
机构信息
Department of Internal Medicine, Ohio State University, Columbus 43210.
出版信息
FEBS Lett. 1993 Mar 1;318(2):139-44. doi: 10.1016/0014-5793(93)80008-i.
A cDNA clone encoding a taurine transporter, designated HTAU, has been isolated from human thyroid. It contains an open reading frame encoding a protein of 619 amino acids with a calculated molecular weight of 69,675 Da. The predicted amino acid sequence of HTAU is highly homologous to those of dog kidney and rat brain. The HTAU mRNA was detectable in various human tissues examined. Transient expression of HTAU in COS-7 cells conferred a higher taurine uptake. The taurine uptake in FRTL-5 cells appears to be regulated by thyrotropin through cAMP. Finally, a higher taurine uptake may be associated with a higher proliferation rate in some cultured cell lines.
一个编码牛磺酸转运体的cDNA克隆，命名为HTAU，已从人甲状腺中分离出来。它包含一个开放阅读框，编码一个由619个氨基酸组成的蛋白质，计算分子量为69,675道尔顿。HTAU的预测氨基酸序列与犬肾和大鼠脑的序列高度同源。在检测的各种人体组织中可检测到HTAU mRNA。HTAU在COS-7细胞中的瞬时表达使牛磺酸摄取增加。FRTL-5细胞中的牛磺酸摄取似乎受促甲状腺激素通过cAMP的调节。最后，在一些培养细胞系中，较高的牛磺酸摄取可能与较高的增殖率有关。
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