一种泛素结合酶RAD6会影响Ty1反转录转座子整合位点的分布。
A ubiquitin-conjugating enzyme, RAD6, affects the distribution of Ty1 retrotransposon integration positions.
作者信息
Liebman S W, Newnam G
机构信息
Department of Biological Sciences, University of Illinois, Chicago 60680.
出版信息
Genetics. 1993 Mar;133(3):499-508. doi: 10.1093/genetics/133.3.499.
Abstract
A galactose-inducible Ty1 element was used to generate 59 independent Ty1 inserts that inactivate the CAN1 gene. As found in previous studies, the distribution of these elements shows a gradient of insertion frequency from highest to lowest between the 5' and 3' ends of the gene. However, 53 independent Ty1 and Ty2 insertions isolated by an identical procedure in an isogenic rad6 deletion strain do not show this bias. In this strain, the Ty elements insert randomly throughout CAN1. These results show that the ubiquitin-conjugating enzyme, RAD6, alters the integration site preferences of Ty1 retrotransposons.
摘要
一个半乳糖诱导型Ty1元件被用于产生59个独立的使CAN1基因失活的Ty1插入片段。如先前研究中所发现的，这些元件的分布在基因的5'端和3'端之间呈现出从最高到最低的插入频率梯度。然而，通过相同程序在同基因rad6缺失菌株中分离得到的53个独立的Ty1和Ty2插入片段却没有显示出这种偏向性。在该菌株中，Ty元件随机插入整个CAN1基因。这些结果表明，泛素结合酶RAD6改变了Ty1逆转座子的整合位点偏好性。
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