丙型肝炎病毒的基因分型

Genotypic subtyping of hepatitis C virus.

作者信息

Chayama K, Tsubota A, Arase Y, Saitoh S, Koida I, Ikeda K, Matsumoto T, Kobayashi M, Iwasaki S, Koyama S

机构信息

Department of Gastroenterology, Toranomon Hospital, Tokyo, Japan.

出版信息

J Gastroenterol Hepatol. 1993 Mar-Apr;8(2):150-6. doi: 10.1111/j.1440-1746.1993.tb01507.x.

DOI:10.1111/j.1440-1746.1993.tb01507.x

PMID:8386022

Abstract

Four subtypes of hepatitis C virus (HCV), Pt(I), K1(II), K2a(III) and K2b(IV), have been suggested based on the nucleotide sequences of the non-structural (NS) 5 region. A fifth subtype from Japanese patients, Tr(V), which shows a less than 68% homology in nucleotide sequence when compared with other subtypes has been identified. A one-step method which enables a quick determination of subtype using polymerase chain reaction with a mixed primer set deduced from the sequence of each subtype has been developed. Using this technique, the subtypes of 418 out of 478 Japanese patients (87.4%) were determined. The incidence of each subtype in Japan was as follows: K1(II), 307 (73.4%); K2a(III), 74 (17.7%); K2b(IV), 28 (6.7%); and Tr(V), 3 (0.7%). This one-step subtyping technique should be useful for studying the epidemiology or biology of the HCV.

摘要

基于非结构（NS）5区的核苷酸序列，已提出丙型肝炎病毒（HCV）的四种亚型，即Pt（I）、K1（II）、K2a（III）和K2b（IV）。已鉴定出一种来自日本患者的第五种亚型Tr（V），与其他亚型相比，其核苷酸序列同源性低于68%。已开发出一种一步法，可使用根据各亚型序列推导的混合引物组通过聚合酶链反应快速确定亚型。使用该技术，确定了478名日本患者中418名（87.4%）的亚型。日本各亚型的发生率如下：K1（II），307例（73.4%）；K2a（III），74例（17.7%）；K2b（IV），28例（6.7%）；Tr（V），3例（0.7%）。这种一步法亚型分析技术对于研究HCV的流行病学或生物学应该是有用的。