Inoue Y, Minasi P, Oh J O
Francis I. Proctor Foundation, University of California, San Francisco 94143-0412.
Invest Ophthalmol Vis Sci. 1993 May;34(6):1954-62.
To study the role of natural killer cells in the dissemination of virus to the eye and virus growth as well as the production of lesions in the eye during primary infection of murine cytomegalovirus.
Virus activity and lesion production by murine cytomegalovirus in various organs of natural killer cell-depleted BALB/c strain of inbred mice were compared with those of immunocompetent normal BALB/c mice.
In mice injected intraperitoneally with murine cytomegalovirus, virus could be isolated from 50% of eyes in the natural killer cell-depleted group whereas no virus was detected from any eye in the control group. In natural killer cell-depleted mice with positive virus isolation from eyes, no murine cytomegalovirus was isolated from either optic nerves or trigeminal ganglia whereas virus was detected from blood lymphocytes, macrophages, granulocytes, and plasma. After intravitreal injection of murine cytomegalovirus, virus titers in eyes of natural killer cell-depleted mice were significantly higher than those in the control group. Derangement of retinal cell layer and inflammatory cells as well as cytomegalic cells in iris and ciliary body were noted in natural killer cell-depleted group, whereas no such changes were observed in the eyes of the control mice.
Natural killer cell depletion enhances the dissemination of murine cytomegalovirus to the eye through the hematogenous route, and increases virus multiplication as well as lesion production in the eye.
研究自然杀伤细胞在鼠巨细胞病毒初次感染期间病毒向眼部传播、病毒生长以及眼部病变产生过程中的作用。
将缺乏自然杀伤细胞的近交系BALB/c小鼠与具有免疫活性的正常BALB/c小鼠进行比较，观察鼠巨细胞病毒在各器官中的病毒活性和病变产生情况。
腹腔注射鼠巨细胞病毒的小鼠中，缺乏自然杀伤细胞组50%的眼睛可分离出病毒，而对照组的任何眼睛均未检测到病毒。在眼睛病毒分离呈阳性的缺乏自然杀伤细胞的小鼠中，视神经或三叉神经节均未分离出鼠巨细胞病毒，而血液淋巴细胞、巨噬细胞、粒细胞和血浆中检测到病毒。玻璃体内注射鼠巨细胞病毒后，缺乏自然杀伤细胞的小鼠眼部病毒滴度显著高于对照组。缺乏自然杀伤细胞组可见视网膜细胞层紊乱、炎性细胞以及虹膜和睫状体中的巨细胞，而对照组小鼠眼睛未观察到此类变化。
自然杀伤细胞缺失可增强鼠巨细胞病毒通过血行途径向眼部的传播，并增加病毒在眼部的增殖以及病变的产生。
