[脂蛋白对血小板中磷脂酰肌醇-4,5-二磷酸和磷脂酸的影响]

[Effect of lipoprotein on phosphatidylinositol -4,5-bisphosphate and phosphatidic acid in platelet].

作者信息

Huang R S, Wang Z

机构信息

Department of Pharmacology, Faculty of Basic Medicine, Peking Union Medical College, Beijing.

出版信息

Zhonghua Yi Xue Za Zhi. 1993 Feb;73(2):85-7, 126.

PMID:8391903

Abstract

Low-density lipoprotein (LDL) and high-density lipoprotein (HDL) were separated from normal human serum and incubated with 32P labelled human platelets. Studies were designed to explore the effects of thrombin, LDL, HDL plus LDL on the changes of the important metabolites: phosphatidylinositol-4,5-bisphosphate (PIP2) and phosphatidic acid (PA) of phosphatidylinositol cycle (PI). The results indicated that both thrombin and LDL caused a significant decrease of PIP2 within 15 seconds. Then, they gradually returned to the control level. This was accompanied simultaneously by a significant increase of PA level for 60 seconds in time-dependent manner. The effects of thrombin or LDL on PIP2 decrease and PA increase were also both dose-dependent. In addition, HDL significantly antagonized the decrease of PIP2 and increase of PA induced by LDL. It is suggested that PI cycle is closely related to atherosclerosis.

摘要

从正常人血清中分离出低密度脂蛋白（LDL）和高密度脂蛋白（HDL），并与32P标记的人血小板一起孵育。设计实验以探究凝血酶、LDL、HDL加LDL对磷脂酰肌醇循环（PI）中重要代谢产物磷脂酰肌醇 - 4,5 - 二磷酸（PIP2）和磷脂酸（PA）变化的影响。结果表明，凝血酶和LDL均在15秒内导致PIP2显著降低。然后，它们逐渐恢复到对照水平。与此同时，PA水平在60秒内以时间依赖性方式显著升高。凝血酶或LDL对PIP2降低和PA升高的影响也均呈剂量依赖性。此外，HDL显著拮抗LDL诱导的PIP2降低和PA升高。提示PI循环与动脉粥样硬化密切相关。