PCR probes for chromosome in situ hybridization of large-insert bacterial recombinants.

We have developed a procedure for efficient in situ hybridization of bacterial recombinants created with various types of large-insert cloning vectors. Minimal quantities of crude DNA are amplified and labeled during the degenerate-oligonucleotide-primed polymerase chain reaction. The resulting probes generate high-intensity fluorescent hybridization signals on metaphase chromosomes and on interphase nuclei.