髓系白血病细胞凋亡过程中28S rRNA可变区V3和V13的选择性切割

Selective cleavage of 28S rRNA variable regions V3 and V13 in myeloid leukemia cell apoptosis.

作者信息

Houge G, Døskeland S O, Bøe R, Lanotte M

机构信息

INSERM U-301, SD1 No 15954.I CNRS, Centre Hayem, Hôpital St. Louis, Paris, France.

出版信息

FEBS Lett. 1993 Jan 2;315(1):16-20. doi: 10.1016/0014-5793(93)81123-h.

DOI:10.1016/0014-5793(93)81123-h

PMID:8416804

Abstract

Vigorous apoptosis is induced 3-4 hours after activation of cAMP-dependent protein kinase I in the rat myeloid leukemia cell line IPC-81 [J. Cell. Physiol. 146 (1991) 73-80]. We will report a novel feature of apoptosis in these cells: a selective and temporarily ordered cleavage within the two largest 28S ribosomal RNA variable regions (V3 and V13). The cleavage of 28S rRNA coincided with internucleosomal DNA fragmentation and cessation of cellular protein synthesis. The implication of 28S variable regions as targets in apoptosis is a clue to the function of these so far apparently superfluous parts of eukaryotic ribosomes.

摘要

在大鼠髓性白血病细胞系IPC - 81中，cAMP依赖性蛋白激酶I激活后3 - 4小时会诱导强烈的细胞凋亡[《细胞生理学杂志》146 (1991) 73 - 80]。我们将报道这些细胞凋亡的一个新特征：在两个最大的28S核糖体RNA可变区（V3和V13）内存在选择性且暂时有序的切割。28S rRNA的切割与核小体间DNA片段化以及细胞蛋白质合成的停止同时发生。28S可变区作为细胞凋亡靶点的意义为真核核糖体这些迄今看似多余部分的功能提供了线索。