D-erythro-sphingosine lowers 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in Chinese hamster ovary cells.

D-Erythro-sphingosine lowered the levels of HMG-CoA reductase activity in CHO-K1 cells. Significant suppression of reductase activity was observed at 5 microM, 10 microM, and 15 microM concentrations of the sphingolipid base and approximately 50% lowering was found at 10 microM. In contrast, L-threo-sphingosine had no effect on the levels of reductase activity under the conditions studied. Direct addition of D-erythro-sphingosine, at concentrations up to 100 microM, to rat liver microsomes had no effect on the levels of HMG-CoA reductase activity. The concentrations of D-erythro-sphingosine required to lower reductase activity in CHO-K1 cells correspond to reported levels of sphingosine in mammalian cells.