Li Q, Whitcup S M, Fujino Y, Nussenblatt R B, Chan C C
Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892.
Invest Ophthalmol Vis Sci. 1993 Jan;34(1):256-9.
To investigate the role of mast cells in the induction of endotoxin-induced uveitis (EIU).
We previously showed that the mean mast cell number in the anterior uvea was greatest for Lewis rats, lowest for Brown Norway (BN) rats, and intermediate for LBNF1 rats. In the current experiment, we assessed the time of onset and severity of EIU in these three rat strains. We then studied changes in mast cell numbers in the anterior uvea during the induction period of EIU.
Time of onset and severity of EIU were related to the number of mast cells in the anterior uvea. EIU occurred earliest in the Lewis rats, and the maximum mean grade of ocular inflammation on a scale of 0 (no inflammation) to 4 (severe inflammation) +/- standard error of the mean was 3.87 +/- 0.13 for Lewis rats, 1.06 +/- 0.06 for BN rats, and 1.19 +/- 0.12 for LBNF1 rats. The difference between the mean grade of inflammation in the Lewis rats and the other two strains was highly statistically significant (P < .001). In the Lewis rats, mast cell numbers +/- SEM decreased from 68.9 +/- 10.8 at baseline to 49.6 +/- 5.9 4 hr after lipopolysaccharide (LPS) injection and to 27.6 +/- 8.4 8 hr after LPS injection, suggesting that mast cell degranulation occurs before the development of EIU.
Mast cells in the anterior uvea appear to potentiate endotoxin-induced ocular inflammation.
研究肥大细胞在内毒素诱导性葡萄膜炎（EIU）诱发过程中的作用。
我们之前发现，前葡萄膜中肥大细胞的平均数量在Lewis大鼠中最多，在棕色挪威（BN）大鼠中最少，而在LBNF1大鼠中介于两者之间。在当前实验中，我们评估了这三种大鼠品系中EIU的发病时间和严重程度。然后，我们研究了EIU诱导期前葡萄膜中肥大细胞数量的变化。
EIU的发病时间和严重程度与前葡萄膜中肥大细胞的数量有关。EIU在Lewis大鼠中最早出现，在0（无炎症）至4（严重炎症）的评分标准下，Lewis大鼠眼部炎症的最大平均等级±平均标准误为3.87±0.13，BN大鼠为1.06±0.06，LBNF1大鼠为1.19±0.12。Lewis大鼠与其他两个品系的炎症平均等级差异具有高度统计学意义（P <.001）。在Lewis大鼠中，肥大细胞数量±SEM从基线时的68.9±10.8降至脂多糖（LPS）注射后4小时的49.6±5.9，以及LPS注射后8小时的27.6±8.4，这表明肥大细胞脱颗粒发生在EIU发展之前。
前葡萄膜中的肥大细胞似乎会增强内毒素诱导的眼部炎症。
