Hodin J, Wistow G
Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
Biochem Biophys Res Commun. 1993 Jan 29;190(2):391-6. doi: 10.1006/bbrc.1993.1060.
Gene recruitment of enzyme crystallins is a novel process in molecular evolution in which genes for some metabolic enzymes acquire extremely high expression in lens without prior gene duplication. Using the RACE method, the 5' end of the mRNAs for duck lens delta 1-, argininosuccinate lyase/delta 2- and lactate dehydrogenase-B/epsilon-crystallin has been amplified from lens and non-lens tissues and sequenced. In all three cases the major transcription start sites were identical in lens and in other tissues. This suggests that for these three genes, and in contrast to at least one other example, gene recruitment does not require the presence of alternative tissue-preferred promoters.
酶晶状体蛋白的基因招募是分子进化中的一个新过程，即一些代谢酶的基因在晶状体中获得极高表达，且无需事先进行基因复制。使用RACE方法，已从鸭晶状体和非晶状体组织中扩增并测序了鸭晶状体δ1-、精氨琥珀酸裂解酶/δ2-和乳酸脱氢酶-B/ε-晶状体蛋白的mRNA的5'端。在所有这三种情况下，晶状体和其他组织中的主要转录起始位点是相同的。这表明，对于这三个基因，与至少一个其他例子相反，基因招募不需要存在组织偏好性的替代启动子。
