Zhao D, Zimmermann A, Wheatley A M
Department of Visceral Surgery, University of Berne, Inselspital, Switzerland.
Hepatology. 1993 Feb;17(2):310-7.
Two models of orthotopic liver transplantation in the rat currently used are with and without reconstruction of the hepatic artery. The aim of this study was to assess the importance of the arterial blood supply to liver structure after orthotopic liver transplantation with advanced morphometric methods. Orthotopic liver transplantation was performed in male Lewis rats, of which 11 underwent reconstruction of the hepatic artery and 11 did not. A group of untreated controls (n = 5) and a group of animals with hepatic artery ligation (n = 4) were included. Eight weeks after surgery liver tissue was harvested and subjected to systematic random sampling. A point-counting method was used, volume fractions of tissue components were determined. Liver samples from rats that underwent hepatic artery reconstruction had preserved lobular architecture, and estimated bile duct (0.71% +/- 0.33% [S.D.]) and connective tissue (1.89% +/- 0.52%) volumes were not significantly different from those of controls (bile duct, 0.34% +/- 0.17%; connective tissue, 0.70% +/- 0.07%). In contrast, liver samples from rats that did not undergo hepatic artery reconstruction showed bile duct proliferation (7.19% +/- 4.83%; p < 0.05) and an increase in connective tissue volume (7.54% +/- 3.68%; p < 0.05) associated with a decrease in hepatocyte volume (controls, 87.3% +/- 0.3%; rats that underwent arterialization, 85.5% +/- 1.0%; rats that did not undergo arterialization, 73.2% +/- 8.2% [p < 0.05]). Interestingly, hepatic artery ligation had no significant effect on any parameter.(ABSTRACT TRUNCATED AT 250 WORDS)
目前在大鼠中使用的两种原位肝移植模型是有和没有肝动脉重建的。本研究的目的是用先进的形态计量学方法评估原位肝移植后动脉血供对肝脏结构的重要性。对雄性Lewis大鼠进行原位肝移植，其中11只进行了肝动脉重建，11只未进行。纳入一组未处理的对照（n = 5）和一组肝动脉结扎的动物（n = 4）。术后8周采集肝组织并进行系统随机抽样。采用点计数法，测定组织成分的体积分数。接受肝动脉重建的大鼠肝脏样本保留了小叶结构，估计的胆管（0.71%±0.33%[标准差]）和结缔组织（1.89%±0.52%）体积与对照无显著差异（胆管，0.34%±0.17%；结缔组织，0.70%±0.07%）。相比之下，未进行肝动脉重建的大鼠肝脏样本显示胆管增生（7.19%±4.83%；p<0.05）和结缔组织体积增加（7.54%±3.68%；p<0.05），同时肝细胞体积减少（对照，87.3%±0.3%；接受动脉化的大鼠，85.5%±1.0%；未接受动脉化的大鼠，73.2%±8.2%[p<0.05]）。有趣的是，肝动脉结扎对任何参数均无显著影响。（摘要截断于250字）
