Haneke E, Schulze H J, Mahrle G
Department of Dermatology, Ferdinand-Sauerbruch-Klinikum, Wuppertal, Germany.
J Am Acad Dermatol. 1993 Feb;28(2 Pt 1):222-6. doi: 10.1016/0190-9622(93)70031-n.
Chromogranin A (CGA) is the major protein of neurosecretory granules (NSG) of cells of the diffuse neuroendocrine system, and the amount of CGA corresponds to the number of NSGs.
Because formalin fixation may destroy NSGs, a study was performed to examine the presence of CGA in Merkel cell carcinoma (MCC) to determine whether CGA depends on the presence of intact NSGs.
Formalin-fixed, paraffin-embedded tissue of 15 MCCs was investigated by immunohistochemistry and immunoelectron microscopy for the presence of CGA and NSGs.
CGA was demonstrated in 12 of 15 MCCs by immunochemistry and in 6 of 10 MCCs by immunoelectron microscopy although intact NSGs could not be discerned in all cases.
CGA remains demonstrable even when no morphologically intact NSGs are present, which suggests that CGA is not exclusively responsible for the electron density of NSGs.
嗜铬粒蛋白A（CGA）是弥漫性神经内分泌系统细胞神经分泌颗粒（NSG）的主要蛋白质，CGA的量与NSG的数量相对应。
由于福尔马林固定可能会破坏NSG，因此进行了一项研究，以检测默克尔细胞癌（MCC）中CGA的存在情况，以确定CGA是否依赖于完整NSG的存在。
通过免疫组织化学和免疫电子显微镜研究15例MCC的福尔马林固定、石蜡包埋组织中CGA和NSG的存在情况。
免疫化学显示15例MCC中有12例存在CGA，免疫电子显微镜显示10例MCC中有6例存在CGA，尽管并非所有病例都能识别出完整的NSG。
即使不存在形态完整的NSG，CGA仍可检测到，这表明CGA并非NSG电子密度的唯一原因。
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