De Libero G, Rocci M P, Casorati G, Giachino C, Oderda G, Tavassoli K, Migone N
Department of Research, University Hospital, Basel, Switzerland.
Eur J Immunol. 1993 Feb;23(2):499-504. doi: 10.1002/eji.1830230230.
In celiac disease large numbers of gamma delta T lymphocytes infiltrate the intestinal epithelia. We have isolated intestinal gamma delta T cell clones from patients with celiac disease and have analyzed their T cell receptor repertoire. T cell lines and clones were obtained from jejunal biopsies of 14 celiac patients and 12 individuals without celiac disease. These were analyzed by staining with monoclonal antibodies against CD3, alpha beta and gamma delta T cell receptor, by Southern blot with gamma- and delta-specific probes and by polymerase chain reaction using V delta-specific oligonucleotides. Intestinal gamma delta cells from patients with celiac disease differed from those of controls with normal jejunal histology in that V delta 1+ cells and V delta 1-V delta 2- cells were significantly increased. There was no evidence of the expansion of one or more clones expressing particular types of gamma delta T cell receptor.
在乳糜泻中，大量γδ T淋巴细胞浸润肠道上皮。我们从乳糜泻患者中分离出肠道γδ T细胞克隆，并分析了它们的T细胞受体库。T细胞系和克隆取自14例乳糜泻患者和12例无乳糜泻的个体的空肠活检组织。通过用抗CD3、αβ和γδ T细胞受体的单克隆抗体染色、用γ和δ特异性探针进行Southern印迹以及使用Vδ特异性寡核苷酸进行聚合酶链反应来分析这些组织。乳糜泻患者的肠道γδ细胞与空肠组织学正常的对照者的肠道γδ细胞不同，在于Vδ1 +细胞和Vδ1 - Vδ2 -细胞显著增加。没有证据表明表达特定类型γδ T细胞受体的一个或多个克隆发生扩增。
