人肾二肽酶基因组DNA的克隆与结构分析

Cloning and structural analysis of genomic DNA for human renal dipeptidase.

作者信息

Satoh S, Kusunoki C, Konta Y, Niwa M, Kohsaka M

机构信息

Product Development Laboratories, Fujisawa Pharmaceutical Co., Ltd., Osaka, Japan.

出版信息

Biochim Biophys Acta. 1993 Feb 20;1172(1-2):181-3. doi: 10.1016/0167-4781(93)90289-p.

DOI:10.1016/0167-4781(93)90289-p

PMID:8439558

Abstract

A genomic DNA for human renal dipeptidase was isolated from a human genomic library using probes for human renal dipeptidase cDNA. The human renal dipeptidase gene, containing ten exons and nine introns, had a total length of approx. 6 kbp. The DNA sequence of these exons was slightly different from that of the human renal dipeptidase cDNA reported by Adachi et al. [1]. From the results of a comparison of the deduced amino acid sequence of each exon with various mammalian renal dipeptidases, the fourth exon was found to be highly conserved (90%).

摘要

利用人肾二肽酶cDNA探针从人基因组文库中分离出了人肾二肽酶的基因组DNA。人肾二肽酶基因包含10个外显子和9个内含子，全长约6kbp。这些外显子的DNA序列与Adachi等人[1]报道的人肾二肽酶cDNA的序列略有不同。通过将每个外显子推导的氨基酸序列与各种哺乳动物肾二肽酶进行比较，发现第四个外显子高度保守（90%）。