Saluz H P, Jost J P
IRBM, Roma, Italy.
Crit Rev Eukaryot Gene Expr. 1993;3(1):1-29.
With the availability of direct genomic footprinting techniques the study of native genomes has been greatly facilitated. This review provides an overview of the techniques involved and gives also a description of the mode of action of different DNA modifying agents which can be used for such methods. These include exonuclease III, deoxyribonuclease, DNase I, micrococcal nuclease, dimethyl sulfate, diethyl sulfate, ethyl methanesulphonate, ethylnitrosourea, diethylpyrocarbonate, bromoacetaldehyde, potassium permanganate, osmium tetroxide, methidiumpropyl-EDTA-iron(II), formaldehyde, psoralen, 1,10 phenanthroline-copper and UV light. We also describe the limitations of the currently existing techniques and give some potential developments.
随着直接基因组足迹技术的出现，天然基因组的研究得到了极大的促进。本综述概述了所涉及的技术，并描述了可用于此类方法的不同DNA修饰剂的作用模式。这些修饰剂包括核酸外切酶III、脱氧核糖核酸酶、DNase I、微球菌核酸酶、硫酸二甲酯、硫酸二乙酯、甲磺酸乙酯、乙基亚硝基脲、焦碳酸二乙酯、溴乙醛、高锰酸钾、四氧化锇、甲基丙基-EDTA-铁（II）、甲醛、补骨脂素、1,10-菲咯啉-铜和紫外线。我们还描述了现有技术的局限性，并给出了一些潜在的发展方向。
