Kumar A, Kurup V P, Greenberger P A, Fink J N
Department of Medicine, Medical College of Wisconsin, Milwaukee 53295-1000.
J Lab Clin Med. 1993 Mar;121(3):431-6.
A hybridoma secreting immunoglobulin G1 antibody was produced against Aspergillus fumigatus. In this study we used interleukin-4 as a growth factor to augment the survival and multiplication of immunoglobulin G1-secreting hybridomas. The antigen recognized by this monoclonal antibody is a low-molecular weight concanavalin A-nonbinding component of A. fumigatus. The monoclonal antibody affinity-purified antigen showed specific reactivity with serum from patients with allergic bronchopulmonary aspergillosis, whereas all allergic asthmatic patients with skin-test reactivity to A. fumigatus demonstrated only low levels of antibodies similar to those in normal control subjects. This antigen failed to show cross-reactivity with other fungal antigens. These results indicate that this monoclonal antibody can be used for the purification of specific antigens useful in the immunodiagnosis of allergic bronchopulmonary aspergillosis.
制备了一种分泌免疫球蛋白G1抗体的杂交瘤，该抗体针对烟曲霉。在本研究中，我们使用白细胞介素-4作为生长因子，以提高分泌免疫球蛋白G1的杂交瘤的存活率和增殖能力。该单克隆抗体识别的抗原是烟曲霉的一种低分子量伴刀豆球蛋白A非结合成分。经单克隆抗体亲和纯化的抗原与变应性支气管肺曲霉病患者的血清显示出特异性反应，而所有对烟曲霉皮肤试验呈反应性的变应性哮喘患者仅显示出与正常对照受试者相似的低水平抗体。该抗原与其他真菌抗原未显示交叉反应。这些结果表明，该单克隆抗体可用于纯化对变应性支气管肺曲霉病免疫诊断有用的特异性抗原。
