Yan M S, Xiue S, Wei L X, Piao X H
Department of Microbiology, Beijing Medical Institute, China.
Immunopharmacol Immunotoxicol. 1993 Jan;15(1):79-85. doi: 10.3109/08923979309066935.
Norcantharidin (NCTD) is a synthetic analog of cantharidin. It has potent antitumor properties without obvious side effect that Cantharidin has on urinary organs. It has been reported that NCTD is an immunological stimulator to NK, LAK, neutrophil and lymphocyte. In our experiment, however, we observed that NCTD can markedly inhibit lymphocyte proliferation stimulated by mitogen ConA or LPS in vitro, and the mixed lymphocyte reaction (MLR) of mice, in a dose-related manner. This occurred even when the drug was added 40 hours (for lymphocyte proliferation) or 72 hours (for MLR) after the cultures were initiated. On the other hand, NCTD has no effect on inactive lymphocytes that were cultured as control in medium without mitogens, suggesting that NCTD selectively acts on activated lymphocyte.
去甲斑蝥素（NCTD）是斑蝥素的合成类似物。它具有强大的抗肿瘤特性，且没有斑蝥素对泌尿器官所产生的明显副作用。据报道，NCTD是一种对自然杀伤细胞（NK）、淋巴因子激活的杀伤细胞（LAK）、中性粒细胞和淋巴细胞有作用的免疫刺激剂。然而，在我们的实验中，我们观察到NCTD能够在体外以剂量相关的方式显著抑制由丝裂原刀豆蛋白A（ConA）或脂多糖（LPS）刺激的淋巴细胞增殖以及小鼠的混合淋巴细胞反应（MLR）。即使在培养开始40小时（针对淋巴细胞增殖）或72小时（针对MLR）后添加该药物，这种情况依然会发生。另一方面，NCTD对在无丝裂原的培养基中作为对照培养的静止淋巴细胞没有影响，这表明NCTD选择性地作用于活化的淋巴细胞。
