嗜热栖热菌过量产生的延伸因子Tu参与大肠杆菌的蛋白质生物合成。

Participation of the overproduced elongation factor Tu from Thermus thermophilus in protein biosynthesis of Escherichia coli.

作者信息

Zeidler W, Kreutzer R, Sprinzl M

机构信息

Laboratorium für Biochemie, Universität Bayreuth, Germany.

出版信息

FEBS Lett. 1993 Mar 15;319(1-2):185-8. doi: 10.1016/0014-5793(93)80064-2.

DOI:10.1016/0014-5793(93)80064-2

PMID:8454054

Abstract

The influence of the overproduced elongation factor Tu (EF-Tu) from Thermus thermophilus on the protein biosynthesis in Escherichia coli was investigated both in vivo and in vitro. A kirromycin-resistant E. coli strain became sensitive to this antibiotic upon the expression of the tuf A-gene of T. thermophilus present on a plasmid. In in vitro translation with components of the kirromycin-resistant E. coli strain the poly(Phe) synthesis stopped when minute amounts of the EF-Tu from T. thermophilus were added. Both results indicate the sensitivity of the T. thermophilus EF-Tu to kirromycin and its participation in the polypeptide synthesis of E. coli.

摘要

研究了嗜热栖热菌过量产生的延伸因子Tu（EF-Tu）对大肠杆菌蛋白质生物合成的体内和体外影响。携带嗜热栖热菌tuf A基因的质粒在抗奇霉素的大肠杆菌菌株中表达后，该菌株对这种抗生素变得敏感。在用抗奇霉素大肠杆菌菌株的成分进行体外翻译时，添加微量嗜热栖热菌的EF-Tu后，聚（苯丙氨酸）合成停止。这两个结果均表明嗜热栖热菌的EF-Tu对奇霉素敏感，并参与了大肠杆菌的多肽合成。