Ferrali M, Signorini C, Ciccoli L, Comporti M
Istituto di Patologia Generale, Università di Siena, Italy.
FEBS Lett. 1993 Mar 15;319(1-2):40-4. doi: 10.1016/0014-5793(93)80033-q.
The incubation of a ghost-free erythrocyte lysate with the oxidizing agent phenylhydrazine resulted in both methemoglobin formation and release of iron in a desferrioxamine (DFO)-chelatable form. The released iron was diffusible, as shown by a dialysis carried out simultaneously with the incubation. When the dialysate was added to erythrocyte ghosts or to microsomes from liver or brain, lipid peroxidation developed in the membranes, indicating that the diffusible iron was in a redox active form. The addition of ATP to the lysate markedly increased both iron diffusion and lipid peroxidation in the membranes subsequently added to the dialysate. The possible implication of these data in some well known pathologies is discussed.
将无幽灵红细胞裂解物与氧化剂苯肼一起孵育，会导致高铁血红蛋白的形成以及以去铁胺（DFO）可螯合形式释放铁。释放出的铁是可扩散的，这在与孵育同时进行的透析中得到了证明。当将透析液添加到红细胞幽灵或肝脏或大脑的微粒体中时，膜中会发生脂质过氧化，这表明可扩散的铁处于氧化还原活性形式。向裂解物中添加ATP会显著增加随后添加到透析液中的膜中铁的扩散和脂质过氧化。讨论了这些数据在一些知名病理学中的可能含义。
