Zeisig R, Jungmann S, Arndt D, Schütt A, Nissen E
Max Delbrück Center for Molecular Medicine, Berlin-Buch, Germany.
Anticancer Drugs. 1993 Feb;4(1):57-64. doi: 10.1097/00001813-199302000-00008.
We investigated the liposome forming properties of three homologues of alkylphosphocholines: hexadecylphosphocholine (HPC), octadecylphosphocholine (OPC) and eicosanylphosphocholine (EPC). In the presence of cholesterol and dicetylphosphate, alkylphosphocholines form liposomes with slow permeability for entrapped carboxyfluorescein. We studied the direct cytotoxicity of alkylphosphocholine vesicles and their ability to attack MethA sarcoma cells, human skin and muscle fibroblasts (M22, GUS, Moscow), and human mouth epidermoid carcinoma cells (KB, ATCC, CCL 17). All alkylphosphocholines show cytotoxic activity against the investigated cells, the degree of which depends on the number of carbon atoms in the alkyl chain, concentration and incubation time. Whereas the etherlipid liposomes are less toxic to MethA cells than the free compounds, the liposomal alkylphosphocholines are more toxic toward KB and M22 cells than the corresponding free lipids.
十六烷基磷胆碱（HPC）、十八烷基磷胆碱（OPC）和二十烷基磷胆碱（EPC）。在胆固醇和十六烷基磷酸存在的情况下，烷基磷胆碱形成对包封的羧基荧光素具有缓慢渗透性的脂质体。我们研究了烷基磷胆碱囊泡的直接细胞毒性及其攻击MethA肉瘤细胞、人皮肤和肌肉成纤维细胞（M22、GUS、莫斯科）以及人口腔表皮样癌细胞（KB、ATCC、CCL 17）的能力。所有烷基磷胆碱对所研究的细胞均表现出细胞毒性活性，其程度取决于烷基链中的碳原子数、浓度和孵育时间。虽然醚脂质体对MethA细胞的毒性低于游离化合物，但脂质体烷基磷胆碱对KB和M22细胞的毒性高于相应的游离脂质。
