Ohsugi K, Ide H
Biological Institute, Tohoku University, Sendai, Japan.
Dev Biol. 1993 Apr;156(2):418-25. doi: 10.1006/dbio.1993.1089.
Local application of retinoic acid (RA) to the anterior-distal margin of chick limb buds causes digit pattern duplications identical to those that result from zone of polarizing activity (ZPA) grafts. The AV-1 antigen is a cell membrane glycoprotein which is expressed during chick limb bud development in a stage- and position-specific manner that correlates with cartilage pattern formation. In order to further understand the limb patterning mechanisms affected by RA and by ZPA grafts, we first examined the effects of RA treatment on AV-1 expression. We then compared the effect of RA with those of ZPA grafts. When beads soaked in varying concentrations of RA were applied to the anterior-distal margin of wing buds, changes in the pattern of AV-1 antigen expression were observed. AV-1 expression appears to correlate with regions that will give rise to a digit 3. These results suggest that the AV-1 protein is one of the molecules which may be involved in the process of cartilage pattern formation during limb development. ZPA grafts produced similar alterations in AV-1 expression, but the initial changes were observed earlier with ZPA grafts than with RA beads. These results support the idea that RA changes anterior limb bud cells into ZPA cells.
将视黄酸（RA）局部应用于鸡胚肢芽的前远端边缘会导致指型重复，这与极化活性区（ZPA）移植所产生的指型重复相同。AV-1抗原是一种细胞膜糖蛋白，在鸡胚肢芽发育过程中以阶段和位置特异性的方式表达，这种方式与软骨模式形成相关。为了进一步了解受RA和ZPA移植影响的肢体模式形成机制，我们首先研究了RA处理对AV-1表达的影响。然后我们将RA的作用与ZPA移植的作用进行了比较。当将浸泡在不同浓度RA中的珠子应用于翼芽的前远端边缘时，观察到AV-1抗原表达模式的变化。AV-1表达似乎与将发育为第3指的区域相关。这些结果表明，AV-1蛋白是可能参与肢体发育过程中软骨模式形成的分子之一。ZPA移植在AV-1表达上产生了类似的变化，但ZPA移植比RA珠子更早观察到初始变化。这些结果支持RA将前肢芽细胞转变为ZPA细胞这一观点。
