Reichart M, Lederman H, Har-Even D, Kedem P, Bartoov B
Department of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
Andrologia. 1993 Mar-Apr;25(2):59-66. doi: 10.1111/j.1439-0272.1993.tb02683.x.
It was suggested that although not related to the standard semen parameters the level of the acrosin enzyme system is related to the fertility potential in men. Recently a simple clinical assay for total acrosin level was recommended for routine semen analysis. The improved clinical assay was analysed on the freshly liquified semen of 198 Asthenoteratozoospermic men and compared with the routine semen parameters including biochemical data and the ultrastructure of the acrosome. Only the sum of the per cent of live, motile, and normal-shaped spermatozoa had positive significant and reasonably high correlation with the acrosin level (r = 0.382, P < 0.0001). Each characteristic exhibits significant but low (< 0.35) correlation. Similarly negative significant and reasonably high correlation was obtained between the acrosin level and the sum of the principle acrosomal malformations observed by TEM (r = 0.396, P < 0.0001) while lower negative correlation was found only with agenesis or loss of the acrosome. Acrosin levels below 8.1 microIU 10(-6) cells were obtained in 4 specimens with above 80% round-form associated with more than 95% of agenesis of the acrosome. The possible significance of the low correlation obtained between the acrosin levels and seminal plasma zinc levels, malformations in the acrosomal equatorial region, and the presence of white blood cells is also discussed. We concluded that the acrosin activity reflects an aspect of male fertility which is not diagnosed by the routine semen analysis or by the ultrastructure of the acrosome, and is therefore a useful diagnostic sperm parameter.
有人提出，尽管顶体酶系统水平与标准精液参数无关，但它与男性的生育潜力有关。最近，有人推荐一种简单的总顶体酶水平临床检测方法用于常规精液分析。对198例弱畸精子症男性新鲜液化精液进行了改进的临床检测，并与包括生化数据和顶体超微结构在内的常规精液参数进行了比较。只有活精子、活动精子和正常形态精子的百分比总和与顶体酶水平呈显著正相关且相关性较高（r = 0.382，P < 0.0001）。每个特征的相关性虽显著但较低（< 0.35）。同样，顶体酶水平与透射电镜观察到的主要顶体畸形总和之间呈显著负相关且相关性较高（r = 0.396，P < 0.0001），而仅与顶体缺失或发育不全呈较低的负相关。在4个标本中，顶体酶水平低于8.1微国际单位/10(-6)个细胞，其中圆形精子比例超过80%，顶体发育不全比例超过95%。还讨论了顶体酶水平与精浆锌水平、顶体赤道区畸形以及白细胞存在之间低相关性的可能意义。我们得出结论，顶体酶活性反映了男性生育能力的一个方面，这是常规精液分析或顶体超微结构所无法诊断的，因此是一个有用的精子诊断参数。
