Braga P C, Dal Sasso M, Bernini A, Bartucci F, Pollo A, Carbone E
Department of Pharmacology, School of Medicine, University of Milan, Italy.
Neurosci Lett. 1993 Mar 5;151(1):85-8. doi: 10.1016/0304-3940(93)90052-m.
Experimental and clinical evidence testifies to an antinociceptive action of salmon calcitonin (sCT), administered in different ways, on the central nervous system. These studies were performed almost exclusively in acute pain models. The purpose of the present study was to investigate the effects of sCT, injected directly into the lateral cerebral ventriculi, on the firing of single nociceptive thalamic neurons, detected by electrophysiological techniques in an experimental model of prolonged or chronic pain, such as rats rendered arthritic by injection of Freund's adjuvant into the left hindfoot. The noxious test stimuli used were either extension or flexion of the ankle or mild lateral pressure on the heel. With increasing doses of sCT (5, 10, 20, 40 micrograms, 5 microliters/i.c.v.) it was possible to observe correspondingly increasing inhibitory and long-lasting effects on the evoked firing, with a significant dose-effect relationship. In agreement with electrophysiological findings, preliminary data, obtained with a patch clamp technique, on depression of calcium fluxes through neuronal membrane, induced by sCT, oriented the attention to a direct action of sCT on CNS.
实验和临床证据证实，以不同方式给药的鲑鱼降钙素（sCT）对中枢神经系统具有抗伤害感受作用。这些研究几乎完全在急性疼痛模型中进行。本研究的目的是通过电生理技术，在诸如通过向大鼠左后足注射弗氏佐剂使其患关节炎的长期或慢性疼痛实验模型中，研究直接注入大脑侧脑室的sCT对单个伤害性丘脑神经元放电的影响。所使用的有害测试刺激为踝关节伸展或屈曲，或足跟轻度侧压。随着sCT剂量增加（5、10、20、40微克，5微升/脑室内注射），可以观察到对诱发放电的抑制作用相应增强且持续时间延长，存在显著的剂量效应关系。与电生理结果一致，用膜片钳技术获得的关于sCT诱导神经元膜钙通量降低的初步数据，使人们将注意力转向sCT对中枢神经系统的直接作用。
