[中间芽孢杆菌7P核糖核酸酶中的离子对]
[Ionic pairs in Bacillus intermedius 7P ribonuclease].
作者信息
Grishina I B, Makarov A A, Kuznetsov D V, Vasil'ev D G, Palovskiĭ A G, Aĭzenmenger F, Esipova N G
出版信息
Biofizika. 1993 Jan-Feb;38(1):22-30.
On the basis of X-ray determined coordinates specified with computer graphics and new data on amino acids sequence in the structure of Bacillus intermedius 7P ribonuclease there were analysed potentially charged residues and found ionic pairs binding independently denaturing regions-energetic domains. Five clusters of charged amino acids were found in the protein structure and several ionic bonds between the energetic domains were discovered: Asp7, Asp11-Arg109 and Lys26-Asp53,Glu72. Breaking of the latter makes their heat denaturation independent. Local surrounding of the ionic bonds is characterized and their role in the structure and cooperative properties of the globule is discussed.
根据通过计算机图形学指定的X射线确定的坐标以及中间芽孢杆菌7P核糖核酸酶结构中氨基酸序列的新数据，分析了潜在带电残基，并发现了结合独立变性区域——能量结构域的离子对。在蛋白质结构中发现了五组带电荷的氨基酸，并发现了能量结构域之间的几个离子键：Asp7、Asp11-Arg109和Lys26-Asp53、Glu72。后者的断裂使其热变性相互独立。对离子键的局部环境进行了表征，并讨论了它们在球状体结构和协同性质中的作用。
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