Schenker E L, Hultin L E, Bauer K D, Ferbas J, Margolick J B, Giorgi J V
Department of Medicine, UCLA School of Medicine 90024.
Cytometry. 1993;14(3):307-17. doi: 10.1002/cyto.990140311.
A basic immunophenotyping panel that employed dual-color combinations of fluorescein isothiocyanate (FITC) and phycoerythrin (PE) conjugated monoclonal antibodies (mAb; FITC-CD45/PE-CD14, FITC-IgG1/PE-IgG2, FITC-CD3/PE-CD8, FITC-CD3/PE-CD4, FITC-CD3/PE-CD16 + PE-CD56, and PE-CD19) was utilized in a quality assurance program to determine whether the 4 laboratories participating in a multicenter AIDS study obtained similar lymphocyte subset percentage values for T cells, B cells, NK cells, and CD4+ and CD8+ T cells. Over a 1 1/2 year period, 78 shared peripheral blood specimens were prepared and analyzed in each laboratory. The CD45bright CD14- percentage for each specimen was used to correct that individual's lymphocyte subset values. Interlaboratory coefficients of variation (CV) for the human immunodeficiency virus type I (HIV) seronegative (n = 38) and HIV-seropositive (n = 40) specimens using this panel were < 3% for total T cells; < 5% for CD4+ T cells and CD8+ T cells; < or = 17% for B and NK cells; and < 8% for CD4T/CD8T ratios. The 6-tube basic immunophenotyping panel has several notable features: a) for clinical studies, it permits comprehensive evaluation of an individual's major lymphocyte subsets, i.e., T, B, NK, and CD4+ and CD8+ T cells; b) for interlaboratory proficiency testing programs, it allows the detection of differences among laboratories in measurements of several functionally distinct cell populations; and c) for within-sample quality assurance, it provides several quality control checks, including the lymphosum, i.e., the sum of an individual's corrected T+B+NK values, a sum that was generally 100 +/- 5% on the HIV-seronegative specimens analyzed in this study.
在一项质量保证计划中，使用了一个基本免疫表型分析组合，该组合采用异硫氰酸荧光素（FITC）和藻红蛋白（PE）偶联的单克隆抗体（mAb）的双色组合（FITC-CD45/PE-CD14、FITC-IgG1/PE-IgG2、FITC-CD3/PE-CD8、FITC-CD3/PE-CD4、FITC-CD3/PE-CD16 + PE-CD56以及PE-CD19），以确定参与一项多中心艾滋病研究的4个实验室是否能获得关于T细胞、B细胞、NK细胞以及CD4+和CD8+ T细胞的相似淋巴细胞亚群百分比值。在1年半的时间里，每个实验室制备并分析了78份共享的外周血标本。每个标本的CD45bright CD14-百分比用于校正该个体的淋巴细胞亚群值。使用该组合对I型人类免疫缺陷病毒（HIV）血清阴性（n = 38）和HIV血清阳性（n = 40）标本进行实验室间变异系数（CV）分析，结果显示：总T细胞< 3%；CD4+ T细胞和CD8+ T细胞< 5%；B细胞和NK细胞<或= 17%；CD4T/CD8T比值< 8%。这个6管基本免疫表型分析组合有几个显著特点：a）对于临床研究，它允许对个体的主要淋巴细胞亚群，即T细胞、B细胞、NK细胞以及CD4+和CD8+ T细胞进行全面评估；b）对于实验室间能力验证计划，它能检测出不同实验室在测量几个功能不同的细胞群体时的差异；c）对于样本内质量保证，它提供了多项质量控制检查，包括淋巴细胞总和，即个体校正后的T + B + NK值之和，在本研究分析的HIV血清阴性标本中，这个总和通常为100 +/- 5%。
